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產(chǎn)品特點

· Highly efficient gene editing in hiPSCs—use either electroporation or gesicles to deliver Cas9 protein and sgRNA with no genomic integration and reduced off-target effects

· Superior single-cell survival—edited hiPSCs exhibit high (typically ~50%) survival when seeded as single cells in a 96-well plate

· Maintenance of pluripotency after editing and during single-cell cloning—hiPSCs maintain high levels (>90%) of pluripotency markers Oct-4, TRA-1-60, and SSEA-4

· Stable karyotype from start to finish—maintain a normal and stable karyotype throughout editing, single-cell cloning, and expansion

· Flexibility to perform gene editing experiments your way—choose from complete kits that provide editing and single-cell cloning solutions using either electroporation-based or utilize the culture media kit (Code No. Y30021) to perform efficient single-cell cloning following your own editing experiments

· Continuous use of DEF-CS technology throughout gene editing/single-cell cloning experiments—use the Cellartis DEF-CS 500 Culture System (Code No. Y30010) before and during gene editing experiments, then during scale-up after single-cell cloning experiments

產(chǎn)品組成

Cellartis® iPSC Single-Cell Cloning DEF-CS™ Culture Media Kit

（Code No. Y30021，1 kit）

· Cellartis DEF-CS 500 Basal Medium (Code No. Y30011; 500 ml)

· Cellartis iPSC Single-Cell Cloning DEF-CS COAT-1 (Code No. Y30018; 2 × 800 μl)

· Cellartis iPSC Single-Cell Cloning DEF-CS Additives

   (Code No. Y30019; 2 × 750 μl, 1 × 500 μl, 1 × 500 μl)

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Takara代理商——華雅思創(chuàng)生物

上海華雅思創(chuàng)生物科技有限公司是一家為生命科學(xué)領(lǐng)域內(nèi)的科學(xué)研究、細胞 和生物制藥相關(guān)人員提供 產(chǎn)品和服務(wù)的生物高科技公司，干細胞 產(chǎn)品、細胞培養(yǎng)試劑耗材、臍帶MSC間充質(zhì)干細胞培養(yǎng)基、IPS細胞ES胚胎干細胞培養(yǎng)基、DC細胞淋巴細胞無血清培養(yǎng)基、R&D細胞因子等種類豐富的產(chǎn)品；涉及HY StemCell、StemRD、CellGenix、PeproTech、Corning、Thermo、Gibco、 PALL、LONZA、BD等國際公司品牌和生產(chǎn)廠家。以“Yes Service"核心服務(wù)理念，緊緊圍繞“Yes Service focus on Biology 服務(wù) 專注生物"打造中華民族全新服務(wù)體系，我們與客戶攜手一起探索生命科學(xué)奧秘，解決人類健康問題，發(fā)展綠色環(huán)保生態(tài)，推動 和諧持續(xù)發(fā)展。

產(chǎn)品推薦：

品牌                貨號                     名稱

CellArtis           Y40002            NDiff® 227

CellArtis           Y40000            RHB-Basal®

CellArtis           Y40001            RHB-A®

CellArtis           Y30010            Cellartis® DEF-CS™ 500 Culture System

CellArtis           Y30021            Cellartis iPSC Single-Cell Cloning DEF-CS™ Culture Media Kit

CellArtis           Y30045            Cellartis® DEF-CS™ 500 Xeno-Free Culture Medium w/o Antibiotics

CellArtis           Y30047            Cellartis® DEF-CS™ 500 Xeno-Free 3D Spheroid Culture Medium w/o antibiotics

CellArtis           Y30071          Cellartis® DEF-CS™ 500 Xeno-Free GMP Grade Basal Medium

人 iPS細胞基因編輯和單細胞克隆系統(tǒng) 現(xiàn)貨供應(yīng)，歡迎咨詢

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